DNA ADDUCT BYPASS POLYMERIZATION BY SULFOLOBUS solfataricus DNA POLYMERASE DPO4
نویسندگان
چکیده
DNA ADDUCT BYPASS POLYMERIZATION BY SULFOLOBUS solfataricus DNA POLYMERASE DPO4 ANALYSIS AND CRYSTAL STRUCTURES OF MULTIPLE BASE-PAIR SUBSTITUTION AND FRAMESHIFT PRODUCTS WITH THE ADDUCT 1,N-ETHENOGUANINE Hong Zang‡, Angela K. Goodenough§¶, Jeong-Yun Choi‡, Adriana Irimia, Lioudmila V. Loukachevitch, Ivan D. Kozekov§, Karen C. Angel‡, Carmelo J. Rizzo§, Martin Egli‡, and F. Peter Guengerich‡** From the Departments of ‡Biochemistry and §Chemistry and ‡§the Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146 Running Title: Replication of Ethenoguanine by DNA Polymerase Dpo4 Address correspondence to: Prof. F. Peter Guengerich, Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, 23 and Pierce Avenues, Nashville, Tennessee 37232-0146, Tel. 615 322-2261; Fax. 615 322-3141; E-mail: [email protected]
منابع مشابه
Calcium is a cofactor of polymerization but inhibits pyrophosphorolysis by the Sulfolobus solfataricus DNA polymerase Dpo4.
Y-Family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus serves as a model system for eukaryotic translesion polymerases, and three-dimensional structures of its complexes with native and adducted DNA have been analyzed in considerable detail. Dpo4 lacks a proofreading exonuclease activity common in replicative polymerases but uses pyrophosphorolysis to reduce the likelihood of incorporat...
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